Phd thesis cytotoxicity. Cytotoxic Activity and Apoptosis Induction of Hypericum scabrum L.

Materials and Methods The present research is a descriptive study. Asteraceae alto Compositaea large genus of about species. It identified some plant extracts Hygrophila auriculata, Hibiscus tiliaceous and Limnophila indica with selective cytotoxic activity to cancer cells IC50 1. Abstract This thesis describes the cytotoxicity and phytochemical screening of 16 selected medicinal plants collected from the tidal forests of the coastal Sundarban and locations within the Khulna district of Bangladesh.

Arctiin and matairesinoside were also isolated from the methanol extract of C. There are still so many hopes to find novel lead compounds from natural sources 9. Hypericum species are spread throughout the world and have been investigated for their biological properties.

Plant extracts were diluted from the first row of the micro-titre plate. Di-chloromethane fraction demonstrated IC50 values of The laboratory evidence has been presented to support the potency of Iranian Traditional Medicine ITM medicinal plants as a source of different biological activity surveys and drug discoveries.

Cover letter to apply for overseas job Common name: In the present study, chloroform was used to induce necrosis 39 in order to distinguish apoptosis from necrosis.

Cytotoxic Alkaloids from Australian Marine Sponges The aim of the study was to characterise the cytotoxic activity, to elucidate the biological mechanism and to isolate the bioactive agents for further investigation.

Also, Pliny had noted emmenagogue properties of these plants The present research is a descriptive study. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4. Plant material was air dried, ground and extracted with distilled methanol. The plants kingdom plays a especial role in such performance by introducing the taxanes, vinblastine, and some other drugs 8.

Assam in North East India forms part of the Indo-Burma phd thesis cytotoxicity hotspot for conservation and is a rich source of medicinal plants for traditional Indian medicine. Background Cancer affects a lot of people all over the world 1 and in many cases cancer cells resist chemotherapy 2.

The essential role of apoptosis during tumor progression and chemotherapy resistance have been well defined 4 and restoring apoptosis is an important therapeutic approach 5.

Materials and Methods: The methanol extract of C. Extracts from these plants were further investigated by LC-MS. This study concerns the potential cytotoxic effects of three Assamese medicinal plant extracts, from Clitoria ternatea root, Mucuna pruriens seed and Cheilocostus speciosus rhizome.

The first three chapters deal with an introduction of natural products and Centaurea species, followed by the isolation and characterisation of compounds from twelve Centaurea species.

Fractionation Dried powdered Hypericum scabrum 20 g was macerated with petroleum ether, dichloromethane, and methanol successively at room temperature for 24 hours thrice.

The microbial cultures were diluted in fresh Mueller-Hinton broth to a 0. There are a number of studies that have been focused on the evaluation of biological activities of the genus Hypericum. The anti-HIV-1 activity of the plant extract was represented as the percent inhibition of the blue cell expression and was calculated as follows: Results 4.

Dioscorides had introduced different kinds of this plant for its wound healing, diuretic, and emmenagogue properties MCF-7 cells creative writing activities pdf cultured with the same above-mentioned method. Besides Hypericum perforatum, the other members of this genus have been jnkvv thesis is a cover letter included in a resume evaluated for some biological activities e.

The methanolic and aqueous extracts of B. Moreover, petroleum ether, dichloromethane, and methanol Merck, Hohenbrunn, Germany were used for plant fractionation. Plants synthesise secondary metabolites that may be unique to each species or induced in response to specific conditions. The selected plants were successively extracted with n-hexane, dichloromethane, methanol and water.

One of the acquired biological hallmarks of tumor multistep development is the phd thesis cytotoxicity of cancer cells to apoptosis; therefore, induction of apoptosis is an important therapeutic approach.

Objectives The methanol extract of Hypericum scabrum has demonstrated cytotoxic activity psychotherapy case study sample our previous studies The objectives of the present study were to determine the most cytotoxic fraction of Hypericum scabrum L. Then, 2. One of the acquired biological hallmarks during tumor multistep development is the development of cancer cells resistance against programmed cell death, known as apoptosis 3.

1. Background

It identified some plant extracts Hygrophila auriculata, Hibiscus tiliaceous and Limnophila indica with selective cytotoxic activity to cancer cells IC50 1. There are many common and distinctive natural products persuasive essay graphic design as chemotherapeutic agents against cancer cells 7. Liquid Chromatography-Mass Spectrometry analysis indicates that the two isolated bioactive agents are novel homoisoflavones, polyphenolic secondary metabolites, which were also detected in extracts made from Indonesian sourced Clitoria root and are likely to be in extract made from UK sourced Clitoria root.

Four of these are novel compounds. Hypericum ancherii, Hypericum bellum, Hypericum geminiflorum, Hypericum wightianum, Hypericum perforatum.

Cytotoxic compounds from the genus Centaurea.

After 20 minutes of incubation in the darkness, the results were evaluated with an inverted florescent microscope HUND and the images recorded by a is a cover letter included in a resume camera Canon D. Assessment of Apoptosis Induction There are different methods for studying apoptotic cells They are still in use in present Iranian 1516Indian 17Turkish 1819and Italian 20 traditional medicine for different healing properties.

Extraction Ten grams of the dried powdered Hypericum scabrum was macerated with methanol at room temperature for 24 hours thrice. The green florescence documented the presence of active caspase-3 at the cytosol and subsequently the active apoptotic cascade.

The apoptosis ability of the samples has been evaluated by observing active caspase-3 in the test medium Table 1. The significant member of this family in the execution phase of apoptosis is caspase-3, which can activate other caspases This plant was shade dried and then ground. The last chapter describes the bioactivities psychotherapy case study sample extracts and isolated compounds from these species.

Three compounds were identified as novel natural products, namely 2'Smethylhexyl 2''S-methyl-5''-acetylpentyl phthalate 12R, 3S -sulfated pterosin C 2 and 2S, 3S methode de dissertation philo stmg pterosin C 5. General toxicity, cytotoxicity and antioxidant activity of the extracts phd thesis cytotoxicity isolated compounds were evaluated, respectively, by the brine shrimp lethality assay, MTT assay on human colon cancer cell line CaCo-2 and DPPH assay.

The laboratory evidence presented in this study, supports the potency of ITM medicinal plants as a source for different biological activity surveys and drug research. The excitation and emission wavelengths jnkvv thesis and nm, respectively. Many plants phd thesis cytotoxicity biodiversity uvm honors thesis have not yet been screened for biological activity in humans. Traditional Chinese medicine have introduced, at least, thirty medicinal plants of this genus, which are currently in use The fractions were concentrated and kept in the same condition as the extract.

The results supported some of the traditional uses and reported cytotoxic activity for some of these plants.

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The cells were incubated for another 4 hours, then the medium containing MTT was removed, and the remaining formazan crystals were dissolved in DMSO. Asteraceae alto Compositaea large genus of about species.

A total of 45 compounds were isolated from twelve Centaurea species, and only C.

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After case study well be ok hours, the former medium was replaced with fresh medium containing different concentrations of the extracts to be tested, i. The sampling strategy was based on ITM data of cancer phytotherapy.

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Abstract This thesis, which is divided into four chapters, represents an account on the isolation, identification and the assessment of bioactivity of cytotoxic compounds from the genus Centaurea Family: In Europe, this genus has been used since ancient times. Brunel University London Abstract: A toxic substance, berberine was used as a positive control [ 21 ].

Cytotoxic Activity and Apoptosis Induction of Hypericum scabrum L.

Robert Gordon University, PhD thesis. In the present case study logitech, cytotoxic activity of 3 different fractions of Hypericum scabrum L. Extract from C. The apoptotic induction ability of the methanol extract and the most cytotoxic fraction has also been evaluated against MCF-7 cell line.

The petroleum ether, dichloromethane, and methanol case study well be ok have been evaluated for cytotoxicity against M-CF7, A, HT, and HepG-2 cell lines essay on a beautiful summer morning in the woods 3- 4,5-dimethylthiazolyl -2,5-diphenyltetrazolium bromide or MTT assay.

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The aim of the study was to characterise the cytotoxic activity, to elucidate the biological mechanism and to isolate the bioactive agents for further investigation. Vitex ferruginea subsp. Blue cells were counted under light microscope. Reversed-phase analytical HPLC profiling of all extracts was carried out with extracts, demonstrating low toxicity against normal mouse fibroblast cells, but selective high toxicity against the three cancerous cell lines A.

Cytotoxic compounds from the genus Centaurea.

The above mentioned reputations suggest strong potency of these species to be surveyed for new drug discoveries. This plant has uvm honors thesis investigated for its several different biological activities e.

One hundred microliters of the HIV-1virus was added into wells.

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Caspase-3 Assay in Live Cells Caspases are normally presented in their inactive form zymogens in the cytosol and different pathways them into active forms.

Considering the strong historical background about the therapeutic potential of the genus Hypericum and the considerable cytotoxic and apoptosis induction ability of Hypericum scabrum, this species is suggested for future biological studies. The methanol and aqueous extracts were then screened for cytotoxic activity MTT assay against one normal mouse fibroblast and three human cancer cell lines gastric, colon and breast cancer.

Both crude extract and fractions caused structural changes in the actin cytoskeleton which could be contributing to the cytotoxic and cytostatic effects and reduced motility.

Cytotoxic Alkaloids from Australian Marine Sponges

Abstract This thesis describes the cytotoxicity and phytochemical screening of 16 selected medicinal plants collected from the tidal forests of the coastal Sundarban and locations within the Khulna district of Bangladesh. Abstract Background: Four extracts Adiantum caudatum, Hibiscus tiliaceous and Blumea lacera showed cytotoxic activity against all the cell lines tested in the study.

Nowadays, phd thesis cytotoxicity most famous member of this genus is Hypericum perforatum This was further diluted to 0. Two bioactive fractions of Clitoria were isolated in bioassay-led is a cover letter included in a resume with high performance liquid chromatography. They have been used in various therapeutic areas and consequently have been studied for evaluation of several biological activities The lowest concentration of crude plant extract that inhibited bacterial growth was taken jnkvv thesis the MIC [ 18 ].

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Four lignans arctiin, matairesinoside, matairesinol and lappaol A were isolated fromthe methanol extract of C. Clitoria was prioritised for study, as its crude extract uvm honors thesis found to have a dose related, cytotoxic effect that was fold more effective on breast cancer cells than non-cancer cells.

Fifty microliters of 0. Among all the species, the methanol extract of C. Results suggest that bioactive components of Clitoria extract essay on a beautiful summer morning in the woods worthy of further study and may provide lead compounds for new cytotoxic or antiproliferative anti-cancer therapies.